SILAC Tissue Culture Media

Category Description

Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC) is a metabolic labelling technique for MS-based quantitative proteomics. Differentially labelled samples are mixed early in the experimental process and analyzed together. The peptide peaks of the differentially labelled samples can accurately quantified relative to each other and used to determine the peptide and protein ratios. Processing and measuring the samples together ensures accurate reproducibility and quantitation.

SILAC labelling requires the cells comprising each sample to be grown in isotopically different tissue culture media. For traditional SILAC, the molecular masses of either the lysine and/or arginine within the media yield media types more commonly referred to as "light", "medium" and "heavy" media. The differences in molecular mass are achieved by the differential use of heavy carbon (13C) and nitrogen (15N) isotopes. This labelling, combined with tryptic digestion of the sample, results in the proteolytic cleavage of proteins at arginine and lysine, thus ensuring that every peptide within the mixture is isotopically labelled.

Dundee Cell Products were the first company in the world to produce ready-to-use SILAC media commercially. Using ready-to-use media saves time and abolishes the risk of contamination; all you need do is add dialyzed serum. DCP produce a wide range of SILAC media , we are also able to produce SILAC media to your specific requirements.

The labelled amino acids use to produce our media have a purity > 99%. The standard means of abbreviating the different amino acid isotopes used within SILAC media are as follows:

R6 = L-Arginine-13C6 HCl
R10 = L-Arginine-13C6, 15N4 HCl
K4 = L-Lysine-4,4,5,5-D4 HCl
K6 = L-Lysine-13C6, HCl
K8 = L-Lysine-13C6,15N2 HCl

Together these are combined to make the different types of media:

R0K0 = unlabelled/ light label
R6K4 = medium label*
R6K6 = medium label*
R10K8 = heavy label

*The type of medium media used depends on how many samples are to be pooled.

Dundee Cell Products also supply a range of SILAC reagents that includes dialyzed foetal bovine and horse serum for SILAC.

 

Name Ref Description Amount  
DMEM-11 LM011

Ready-to-use SILAC DMEM Media containing 13C labelled arginine and lysine amino acids (R6K6) - 500ml

500ml £249.00

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DMEM-14 LM014

Control SILAC DMEM Media containing unlabelled arginine and lysine amino acids (R0K0) - 500ml

500ml £30.00

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DMEM-15 LM015

Ready-to-use SILAC DMEM Media containing 13C and 15N labelled arginine and 13C and 15N labelled lysine (R10K8) - 500ml

500ml £222.00

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DMEM-16 LM016

Ready-to-use SILAC DMEM Media containing 13C labelled arginine and 2D labelled lysine amino acids (R6K4) - 500ml

500ml £204.00

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DMEM-17 LM017

Ready-to-use SILAC DMEM Media containing 13C labelled arginine and 13C and 15N labelled lysine amino acids (R6K8) - 500ml

500ml £240.00

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RPMI-18 LM018

Ready-to-use SILAC RPMI 1640 Media containing 13C labelled arginine and lysine amino acids (R6K6) - 500ml

500ml £199.00

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RPMI-19 LM019

Control SILAC RPMI 1640 Media containing unlabelled arginine and lysine amino acids (R0K0) - 500ml

500ml £35.00

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RPMI-20 LM020

Ready-to-use SILAC RPMI 1640 Media containing 13C labelled arginine and 13C and 15N labelled lysine amino acids (R6K8) - 500ml

500ml £199.00

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RPMI-21 LM021

Ready-to-use SILAC RPMI 1640 Media containing 13C and 15N labelled arginine and 13C and 15N labelled lysine (R10K8) -500ml

500ml £199.00

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RPMI-22 LM022

Ready-to-use SILAC RPMI 1640 Media containing 13C labelled arginine and 2D labelled lysine amino acids (R6K4) - 500ml

500ml £199.00

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L15-24 LM024

Control SILAC Leibowitz L15 Media containing unlabelled arginine and lysine amino acids (R0K0)

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L15-25 LM025

Ready-to-use Leibowitz L15 Media containing 13C labelled arginine and 13C and 15N labelled lysine amino acids (R6K8)

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L15-26 LM026

Ready-to-use SILAC Leibowitz L15 Media containing 13C and 15N labelled arginine and 13C and 15N labelled lysine (R10K8)

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L15-27 LM027

Ready-to-use SILAC Leibowitz L15 Media containing 13C labelled arginine and 2D labelled lysine amino acids (R6K4)

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IMDM-28 LM028

Ready-to-use SILAC Iscove's Modified Dulbecco's Medium (IMDM) containing 13C labelled arginine and lysine amino acids (R6K6)

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IMDM-29 LM029

Control SILAC Iscove's Modified Dulbecco's Medium (IMDM) containing unlabelled arginine and lysine amino acids (R0K0)

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IMDM-30 LM030

Ready-to-use SILAC Iscove's Modified Dulbecco's Medium (IMDM) containing 13C labelled arginine and 13C and 15N labelled lysine amino acids (R6K8)

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IMDM-31 LM031

Ready-to-use SILAC Iscove's Modified Dulbecco's Medium (IMDM) containing 13C and 15N labelled arginine and 13C and 15N labelled lysine (R10K8)

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IMDM-32 LM032

Ready-to-use SILAC Iscove's Modified Dulbecco's Medium (IMDM) containing 13C labelled arginine and 2D labelled lysine amino acids (R6K4)

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WME-33 LM033

Ready-to-use SILAC Williams Medium E containing 13C labelled arginine and lysine amino acids (R6K6)

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WME-34 LM034

Control SILAC Williams Medium E containing unlabelled arginine and lysine amino acids (R0K0)

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WME-35 LM035

Ready-to-use SILAC Williams Medium E containing 13C labelled arginine and 13C and 15N labelled lysine amino acids (R6K8)

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WME-36 LM036

Ready-to-use SILAC Williams Medium E containing 13C and 15N labelled arginine and 13C and 15N labelled lysine (R10K8)

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WME-37 LM037

Ready-to-use SILAC Williams Medium E containing 13C labelled arginine and 2D labelled lysine amino acids (R6K4)

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F12-38 LM038

Control SILAC DMEM/F12 containing unlabelled arginine and lysine amino acids (R0K0) - 500ml

500ml £35.00

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F12-39 LM039

Ready-to-use SILAC DMEM/F12 containing 13C labelled arginine and lysine amino acids (R6K6) - 500ml

500ml £233.00

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F12-40 LM040

Ready-to-use SILAC DMEM/F12 containing 13C labelled arginine and 13C and 15N labelled lysine amino acids (R10K8) - 500ml

500ml £202.00

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F12-41 LM041

Ready-to-use SILAC DMEM/F12 containing 13C and 15N labelled arginine and 13C and 15N labelled lysine (R6K4) - 500ml

500ml £198.00

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F12-42 LM042

Ready-to-use SILAC DMEM/F12 containing 13C labelled arginine and 2D labelled lysine amino acids (R6K8) - 500ml

500ml £222.00

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MEM-71 LM071

Ready-to-use SILAC MEM Media containing 13C labelled arginine and lysine amino acids (R6K6)

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MEM-72 LM072

Control SILAC MEM Media containing unlabelled arginine and lysine amino acids (R0K0)

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MEM-73 LM073

Ready-to-use SILAC MEM Media containing 13C labelled arginine and 13C and 15N labelled lysine amino acids (R6K8)

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MEM-74 LM074

Ready-to-use SILAC MEM Media containing 13C and 15N labelled arginine and 13C and 15N labelled lysine (R10K8)

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MEM-75 LM075

Ready-to-use SILAC MEM Media containing 13C labelled Arg and 2D labelled Lys amino acids (R6K4)

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M-61 LM61

Ready-to-use SILAC M199 Media containing 13C labelled arginine and lysine amino acids (R6K6)

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M-62 LM62

Control SILAC M199 Media containing unlabelled arginine and lysine amino acids (R0K0)

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M-63 LM63

Ready-to-use SILAC M199 Media containing 13C labelled arginine and 13C and 15N labelled lysine amino acids (R6K8)

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M-64 LM64

Ready-to-use SILAC M199 Media containing 13C and 15N labelled arginine and 13C and 15N labelled lysine (R10K8)

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M-65 LM65

Ready-to-use SILAC M199 Media containing 13C labelled arginine and 2D labelled lysine amino acids (R6K4)

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MC-51 MC51

Ready-to-use SILAC McCoy’s Media containing 13C labelled arginine and lysine amino acids (R6K6)

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MC-52 MC52

Control SILAC McCoy’s Media containing unlabelled arginine and lysine amino acids (R0K0)

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MC-53 MC53

Ready-to-use SILAC McCoy’s Media containing 13C labelled arginine and 13C and 15N labelled lysine amino acids (R6K8)

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MC-54 MC54

Ready-to-use SILAC McCoy’s Media containing 13C and 15N labelled arginine and 13C and 15N labelled lysine (R10K8)

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MC-55 MC55

Ready-to-use SILAC McCoy’s Media containing 13C labelled arginine and 2D labelled lysine amino acids (R6K4)

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SCM-43 MI043

Ready-to-use SILAC Schneider's insect medium containing 13C labelled arginine and lysine amino acids (R6K6)

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SCM-44 MI044

Control SILAC Schneider's insect medium containing unlabelled arginine and lysine amino acids (R0K0)

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SCM-45 MI045

Ready-to-use SILAC Schneider's insect medium containing 13C labelled arginine and 13C and 15N labelled lysine amino acids (R6K8)

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SCM-46 MI046

Ready-to-use SILAC Schneider's insect medium containing 13C and 15N labelled arginine and 13C and 15N labelled lysine (R10K8)

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SCM-47 MI047

Ready-to-use SILAC Schneider insect medium containing 13C labelled arginine and 2D labelled lysine amino acids (R6K4)

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