Products: Fluorescent Cell Markers > PSVue™


PSVue™ Fluorescent Kits for Labeling Apoptotic Cells, Bacteria and Other Anionic Membranes

PSVue™ reagents are a family of fluorescent probes containing a bis(zinc2+dipicolylamine) group (Zn-DPA), a motif that has been found to bind with high affinity to surfaces enriched with anionic phospholipids, especially phosphatidylserine (PS) exposed on cell membranes. The fluorescent part of the probe is a reporter element that provides a means of detecting the probe once it is bound to the membrane of interest. 
 

Key Features of PSVue™ Probes:

  • Bind to a variety of cell types which have negatively charged phospholipids exposed on their membranes including apoptotic cells, necrotic cells, Gram + ve and Gram – ve bacteria, activated cells, tumor vascular endothelial cells, viruses, etc.
  • Available in a range of detection wavelengths from long-UV to near infrared.
  • Suitable for in vitro and in vivo use.
  • Suitable for high-throughput screening assays.
  • Bind to the same PS site as annexin-V.

 

Advantages of PSVue™ over Fluorescent Annexin-V:

  • Binding kinetics are fast; annexin-V binding is slow
  • Binding is Ca2+ independent; means no artifacts due to activation of nonspecific membrane scramblases by Ca2+.
  • Cheap compared to most annexin-V fluorescent analogs
  • Apoptosis can be detected under a wide variety of conditions (e.g. in presence of 10% serum, temps from 4 to 37oC).
  • Can provide more intense labeling due to their much smaller size (i.e. >10 PSVueTM molecules can bind to the same area as 1 annexin V molecule).

 

PSVue™ is a trademark of Molecular Targeting Technologies, Inc.
Sold under agreement from MTTI

Name Ref. Description
PSVue™794 P-1001 The PSVue™794 (formerly PSS-794) reagent kit contains components to provide ~ 0.68 mL of a 1 mM solution of PSVue™794 in aqueous solution. The compound exhibits absorbance and fluorescence excitation maximum at 794 nm and emission maximum at 810 nm. The labeling vehicle provided with the kit (Diluent X) is designed to maximize dye solubility and is suitable for in vitro and in vivo use.
Product insert pdf
PSVue™380 P-1002

The PSVue™380 (formerly PSS-380) reagent kit contains components to provide ~ 0.40 mL of a 2 mM solution of PSVue™380 in 50% aqueous ethanol solution. The compound has an absorbance max at 380nm and a fluorescence emission max at 440nm.

Product insert pdf
PSVue™480 P-1003 The PSVue™480 (formerly PSS-480) reagent kit contains components to provide ~0.5 mL of a 1 mM solution of PSVue™480 in 50% aqueous dimethylsulfoxide solution. The compound has an absorbance max at 480nm and an fluorescence emission max at 519 nm
Product insert pdf