Custom Monoclonal Antibody Service

1. The protocol includes immunization of five mice in 3-5 injections (according to the response)

2. We test by dot blotting after 2nd and 3rd injection to determine mice with best response to antigen

3. Fusion of splenocytes and myeloma cells

4. Screening to identify suitable clones after fusion

5. We will discuss with you during the development process the best design for the screening test in order to get the maximum number of hybridomas suitable for the customer's work

6. We can perform small or large-scale production of antibodies for you as well as purify your monoclonal antibody using protein A or protein L columns

Production Schedule

Activity No.	Procedure	Description	Timetable (weeks)
1	Immunization	5 mice immunised using 50µg of antigen (4 injections per mouse)	6-8
2	Cell-Fusion	Fusion of splenocytes and myeloma cells	7 or 9
3	Screening	Screening of hybridomas for production of non-specific Ig and recognition of specific antigen	10
4	Propagation of hybridomas	Cryopreservation and production of cell bank	11
5	Recloning	Recloning of all positive hybridoma cell colonies and Ig (IgG1, IgG2a, etc) characterization	11&12
6	Expansion	Production of 50ml of supernatant from each clone and preparation of cell bank for each cloned hybridoma	12 & 13