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Mass Spectrometry
Dundee Cell Products offers a comprehensive Mass Spectrometry service to meet our customer's needs. Proteomics services offered include;
- SILAC Qantitative Proteomics
- Label-Free Quantitative Proteomics
- iTRAQ (isobaric Tag for Relative and Absolute Quantitation analysis)
- ICAT (Isotope Coded Affinity Tags analysis)
- Phosphorylation Site Mapping
From routine analysis to interpretation of data, our service can be tailored to suit your individual needs. General services offered include;
MALDI-TOF MS(MS) analysis of proteins, peptides, oligonucleotides and oligosaccharides
ESI-MS(MS) analysis of proteins, peptides, oligonucleotides, oligosaccharides and small molecules
Accurate mass determination, mass spectral data interpretation and specialist in-house database generation
Please contact our customer services department in the first instance to discuss your individual requirements.
Related Pages
SILAC Cell Culture Reagents & Labeled Cell Fractions
Phosphorylation Site Mapping
Our state of the art 4000 QTRAP (Applied Biosystems) can be used to identify sites of phosphorylation from a protein in a 1D band or 2D gel spot.
The 1D band or 2D spot must have good intensity by colloidal Coomassie staining in order to proceed; this gives us a better chance of identifying the sites of phosphorylation as the level of protein usually gives no indication of the stoichiometry of the phosphorylation present.
Samples are normally processed and then in-gel digested with trypsin. The digested samples are then extracted and are taken through three stages of analysis using the 4000 QTRAP machine. The stages include;
1. Identification of protein(s) by nLC-MS-MS
2. Detection of phosphorylated peptides
3. Data interpretation and final reporting
Mascot reports of protein identification(s) and identification of sites of phosphorylation are provided electronically or as a hardcopy if requested.